Embryonic development is a remarkably robust process that displays high degrees of adaptation between species. Therefore, it is important to quantify and compare the developmental progression of related species. Light-sheet fluorescent microscopy (LSFM) is routinely used at the AK Stelzer, Buchmann Institute for Molecular Life Sciences, to perform in-vivo 3D imaging of developing insect embryos. Multiple insect species have been recorded at the AK Stelzer, with a focus on Tribolium Castaneum, commonly known as the red flour beetle. Recently we have observed a phenomenon previously undescribed in T. Castaneum - contraction waves of the serosa cells previous to dorsal closure. In order to detect the serosa contraction waves, we performed 3D particle image velocimetry (PIV) analyses with quickPIV, an open source 3D PIV software developed at the AK Matthäus, Frankfurt Institute for Advanced Studies. From the PIV vector fields we found a clear correlation between migration speed and the onset of the contraction waves. In addition, by quantifying the change in the direction of cellular migration during contraction waves, we could locate the position of the waves in time and space. In order to further investigate the contraction waves, we plan to integrate the contraction wave detection algorithms into the recording setup, allowing us to increase the temporal resolution of the microscope to obtain detailed recordings of the contraction waves.